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Products > Classic DNA Ladders & Markers > 500bp ladder(M1251/M1252)

500bp ladder(M1251/M1252)

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Description
The 500bp ladder is ideal for determining the size of double-stranded DNA from 500 to 5,000 base pairs. The ladder consists of 10 linear double-stranded fragments. The 2,000bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ?l loading, all fragments except 2,000bp are 40ng. The 2,000bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use. 

Recommended Loading
2-5 ?l/Lane

Concentration
Typical Bands    100 ng/5?l
Other Bands      40 ng/5?l

Advised Electrophoresis Condition
8 cm, 1% Agarose Gel, 1×TAE, 7 V/cm, 45min
Contents(bp)
500、1000、1500、2000、2500、3000、3500、4000、4500、5000

Mix Concentration
92 ng/?l
 
Storage


Stable for 3 months at RT, for long-time storage, please store at -20℃. 

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