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Products > Loading Buffer > Gel Loading Dye, Three-colour (6X)(M9061)

Gel Loading Dye, Three-colour (6X)(M9061)

Contents
Gel Loading Dye, Three-colour (6X):

10 mM Tris-HCL(pH 7.6)
0.03% Bromophenol Blue
0.03% Xylene Cyanol
0.15% Orange G
60% Glycerol
60 mM EDTA
 
Description
Gel Loading Dye, Three-colour (6X) is a pre-mixed Loading Buffer with three tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue, xylene cyanol and orange G are the standard tracking dye for electrophoresis.

Storage 
Store at RT
Quality Assurance Statement
Gel Loading Dye, Three-colour (6X) is assayed for contaminating endonuclease, exonuclease and RNase activity.
 
Dye Migrate Rate (on a standard 1% TAE or TBE agarose gel) 
Xylene Cyanol
TAE: 4160 bp
TBE: 3030 bp

Bromophenol Blue 
TAE: 370 bp
TBE: 220 bp

Orange G
TAE/TBE<50 bp

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