? HSTM Taq DNA Polymerase has two concentrations with 2.5 U/μl and 5 U/μl, default package is 5 U/μl.
? 10×HSTM PCR Buffer (Mg2+ Plus) can replace with 10×PCR Buffer (Mg2+ free) and 25 mM MgCl2. Please choose the appropriate package for your experiment.
HSTM Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus , its molecular weight is 94 kDa. HSTM Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 0.9~1.2kb/min. It has 5' to 3' polymerase activity but lacks of 3' to 5' exoneclease activity, which results in a 3'-dA overhangs PCR product. All conponents of the HSTM PCR Buffer are at optimal concentration for efficient amplification, it contributes to highly specific incorporation of primer and template.
Highly thermostable -have a half-life of over 40 min at 95°C incubation
Generates 3'-dA overhangs PCR products
Generate PCR product for TA cloning
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests
Functionally tested in PCR
10x HSTM PCR Buffer with Mg2+
200 mM Tris-Cl(PH 8.8), 100 mM KCl, 16 mM MgSO4, 1% Triton-X-100.
20 mM Tris-HCl (pH8.0), 100mM KCl, 3 mM MgCl2 1mM DTT，0.1% NP-40 ,0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol
Definition of Activity Unit
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Store all components at -20°C