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HS Kit(P3082)

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Description

HSTM Kit contains a HSTM Taq DNA polymerase and a 2x premixed, ready-to-use HSTM reaction Mix. The HSTM reaction mix is supplied as a 2X concentrated solution, that contains dNTPs and all other PCR components, except DNA template and primers. To prepare the final PCR reaction system, mix HSTM Taq DNA polymerase and the HSTMreaction mix, then add primer and template. The reaction mix contributes to high specificity by optimizing the system, reducing primer-dimer rate. PCR amplification sensitivity is controllable due to the amount of DNA polymerase is flexible.

HSTM Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus , its molecular weight is 94 kDa. HSTM Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 0.9~1.2kb/min. It has 5' to 3' polymerase activity but lacks of 3' to 5' exoneclease activity, that results in a 3'-dA overhangs PCR product. All conponents of the HSTM Mix are at optimal concentration for efficient amplification, it contributes to highly specific incorporation of primer and template.

Feature

Convenient – Dongseng recombinant Taq DNA Polymerase in a ready-to-use mix. 
Fast -saves time due to reduced number of pipetting steps. 
Reproducible -lower contamination and pipetting error risk. 
Compatible with TA cloning - generates PCR products with 3'-dA overhangs.

Applications

high specific PCR
High throughput PCR. 
high reproducible PCR. 
Generation of PCR products for TA cloning. 

Quality Control

The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.

Composition of the HSTM Mix
50 mM KCl
20 mM Tris-Cl
3 mM MgSO4
0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP).
bromophenol blue
ddH2O

Storage buffer for HSTM Taq DNA polymerase 
20 mM Tris-HCl
1 mM DTT
0.1 mM EDTA
100 mM KCl
0.5 % TW 20
0.5 % NP 40
50 % Glycerol

Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTP’s into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Store at -20°C.
Repeated freeze-thaw cycles do not reduce the activityof the reactions

Protocol for PCR
All solutions should be thawed on ice, gently vortexed and briefly centrifuged.
Add in a thin walled PCR tube on ice:

For a total 50μl reaction volume
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·Gently vortex the sample and briefly centrifuge to collect all drops to the bottom of the tube.
·Overlay the sample with mineral oil or add an appropriate amount of wax. This step may be omitted if the thermal cycler is equipped with a heated lid.
·Place samples in a thermocycler and start the program.
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