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Products > DNA Purification > LE-Agarose 1200(N9051)

LE-Agarose 1200(N9051)

LE-Agarose 1200Catalog #SizePrice
N9051500g

 

Description

LE-Agarose 1200 is a low EEO, high gel strength agarose for analysis of nucleic acid molecules. Typical applications include electrophoretic separation of DNA and RNA, Southern & Northern blotting, and also lmmunodiffuson and Ouchterlony of proteins.

Analytical Specifications
EEO (-m r)0.1-0.15
Water Content≤10%
Sulfate (SO4)0.15-0.2%
Gel Strength (1% gel)≥1200 g/cm2
Gelling Point (1.5% gel)33±1.5oC
Melting Point (1.5% gel)87±1.5oC

Concentrations for DNA separation
The appropriate agarose concentration depends on the size of the DNA fragments to be separated, the buffer used, and the applied voltage. With IX TAE buffer, the suggested concentrations for DNA sizes in base pairs (bp)  follows :

size range (bp)cincentration
800-100000.8%
400-80001.0%
200-40001.5%
100-30002.0%

 


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