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Products > Loading Buffer > Gel Loading Dye, SDS+(6X)(M9081)

Gel Loading Dye, SDS+(6X)(M9081)

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Contents
Gel Loading Dye, SDS+ (6X):

10 mM Tris-HCL(pH 7.6)
0.03% Bromophenol Blue
0.03% Xylene Cyanol
60% Glycerol
1% SDS
100 mM EDTA
 
Description
Gel Loading Dye, SDS +(6X) is a pre-mixed Loading Buffer with two tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis.This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue and xylene cyanol are the standard tracking dye for electrophoresis.

Storage 
Store at RT

 

Quality Assurance Statement
Gel Loading Dye, SDS+ (6X) is assayed for contaminating endonuclease, exonuclease and RNase activity.
 
Dye Migrate Rate (on a standard 1% TAE or TBE agarose gel) 
Xylene Cyanol
TAE: 4160 bp
TBE: 3030 bp

Bromophenol Blue 
TAE: 370 bp
TBE: 220 bp


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