96 Deep-Well Plate Viral RNA/DNA Miniprep Kit (Magnetic Beads)
Catalog No.: V4003 Size: 96 preps
Based on the method of magnetic bead separation and purification, the 96-Well Plate Viral RNA/DNA Miniprep Kit (Magnetic Beads) is suitable for the purification of high quality viral nucleic acid from saliva, swab, serum, plasma, lymphatic fluid, non-cellular body fluid, cell culture supernatant, tissue homogenate or various virus preservation solutions. The kit can be integrated with an automatic nucleic acid extractor for high-throughput extraction experiments, or manually operated with a magnetic separation rack.
The purification system uses superparamagnetic nano-magnetic particles as the matrix, which can adsorb nucleic acids specifically through hydrogen bonds and static electricity under the condition of high concentration of leachate, while proteins and other non-specific impurities are removed by washing. Finally the nucleic acid are eluted with low salt buffer or RNase-free ddH2O. The purified nucleic acid can be used for various routine operations, including RT-PCR, RT-qPCR, fluorescence quantitative PCR (qPCR) and other downstream experiments.
The kit consists of the following components:
Plate-A (Lysis Buffer/Mag Beads)
Plate-B (Wash Buffer)
Plate-C (Wash Buffer)
Plate-D (Elution Buffer)
1 ml × 2
Prepare 1× PBS solution, pH 7.4, in case of need.
The kit can be stored for 12 months at room temperature (15-25°C) under dry conditions.
1. Avoid repeated freezing-thawing of samples, otherwise the extracted viral RNA will be degraded and the extracted amount will decrease. The samples can be extracted immediately or stored at 4°C for testing for no more than 24 hours. Long-term storage can be placed at -20°C or -80°C.
2. All operating procedures, if not specified, are carried out at room temperature (15-25°C).
3. When using this kit, please wear lab coat, disposable latex gloves, disposable masks and use RNase-free consumables to avoid RNase pollution to the greatest extent. Prepare your own RNase-free pipette tips, etc.
4. Please read the instructions carefully before use and follow the instructions strictly. Clinical samples should be carried out in the ultra clean table or biosafety cabinet.
5. There may be residual magnetic beads during elution, magnetic beads should be avoided as far as possible when pipetting samples.
6. The virus has a strong ability to infect, a variety of defense measures must be done before the operation. Proper disposal of samples and reagent materials, thorough cleaning and disinfection of the operating table.
A. Throat swab (with preservation solution), saliva: vortex vigorously for 30 sec, take 200 ul for experiment.
B. Plasma, serum and viral stock solution: prepare 10-200 ?l of plasma, serum or viral stock solution, if the initial amount is less than 200 ?l, use PBS solution to make up to 200 ?l.
C. Virus-infected tissue: prepare 10 mg of virus-infected tissues to be ground with liquid nitrogen, and add 200 ?l of PBS solution to the ground tissues.
Protocol: Automatic Operation Process of 96 Deep-Well Plate
1. Take out the pre-packaged 96 deep-well plates.
2. Oscillate four angles of the Plate-A to make the Mag Beads suspended.
3. Open the sealing membranes of the 96 deep-well plates.
4. Add 20 ?l Proteinase K and 200 ?l sample to each well of the Plate-A.
5. Place the Magnetic Head into the Plate-C.
6. Follow the instructions to put the 4 pre-packaged plates into the correct position of the machine.
7. Run the viral nucleic acid extraction program of magnetic bead method “gdsbio_normal_96.bdz”, or refer to the “Sheet of Program Design” below.
8. At the end of the automation process, in the Plate-D is the DNA/RNA solution, sealed with a sealing membrane and stored at -20°C or -80°C.
Sheet of Program Design
Mixing at 20~55°C for 10 min by vibrating, and the digested sample release DNA/RNA to the Mag Beads. Incubate for 2 min.Tansfer the beads to Plate-B.
Wash the beads by vibrating for 2 min, then tansfer the beads to Plate-C.
Wash the beads by vibrating for 2 min, dry the beads for 2 min, then tansfer the beads to Plate-D.
Elute the beads by vibrating for 2 min, then tansfer the beads back to Plate-C.
Thermo KingFisher Flex96, Allsheng Auto-Pure96 Plus, etc