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Marker12

Quick Details

  • CAS No.: 100403-24-5

  • EINECS No.: 231-791-2

  • Place of Origin: Guangdong, China (Mainland)

  • Purity: 75ng/ul

  • Classification: High Purity Reagents

  • Brand Name: DSB

  • Model Number: M1141/M1142

  • Color: Blue

Packaging & Delivery

Packaging DetailsItem No: M1141 
Size: 50ug 
Item No: M1142 
Size: 5x50ug 
Pack in vials
Delivery Time1-2 weeks

Concentration: 75ng/?l         

 

Recommended Loading

2-5 ?l/Lane.

 

Concentration

Typical Bands     125 ng/5?l

Other Bands      50 ng/5?l

 

Recommended Electrophoresis Condition

5 ?l/Lane, 8 cm 1.7 %Agarose Gel, 0.5×TBE, 5V/cm, 1h.

 

Contents(bp)

200 ,400,750,1,000,1,500,2,000

 

Storage

1 year at room temperature, for long-time preservation, please storage at -20°C .

 

Description

Marker 12 is ideal for determining the size of double-stranded DNA from 200 to 2,000 base pairs. The ladder consists of 6 linear double-stranded fragments .The 1,000bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 1,000bp are 50ng. The 1,000bp fragment is 125ng. This ladder is pre-mixed with loading dye and is ready to use.  

 

Recommendations

Do not heat before loading.

For quantification, adjust the concentration of the sample to equalize

it approximately with the amount of DNA in the nearest band of the ladder.

Visualize DNA by staining with ethidium bromide or with SYBR? Green I.

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